Posts Tagged: disease

UC Davis to build new $5.25M greenhouse to protect U.S. grapevine collection

Project designed to prevent red blotch and other grapevine diseases

A new, $5.25 million greenhouse is being built on the University of California, Davis, campus to safeguard an important grapevine collection from red blotch disease and other pathogens. 

The 14,400-square-foot greenhouse will have a vestibuled entry, be insect-proof and provide another level of disease protection. It is being spearheaded by Foundation Plant Services, or FPS, which provides the U.S. grape industry with high-quality, virus-tested grapevine plant material.

The program serves as the primary source for grapevine plant material distributed to nurseries under the California Department of Agriculture's Grapevine Registration and Certification Program, which provides the majority of grapevines planted in the United States. For the grape industry, it is essential to protect this material from disease-carrying insects and guarantee fast access to clean plant material.

“The program is considered the largest quarantine center for the grapevine industry in the United States,” said Maher Al Rwahnih, a plant pathologist and FPS director. “This is kind of a game changer for us.”

A history of serving the grapevine industry

FPS has maintained healthy grapevine planting stock on the UC Davis campus for more than 70 years in open fields at the Classic and Russell Ranch foundation vineyards. FPS scientists first detected grapevine red blotch virus at Russell Ranch in 2017. By 2021, an estimated 51.6% of the crop there was infected. Material from that vineyard is not being sold, and the site is now part of an epidemiological study to try to pinpoint how the disease is transmitted.

FPS pathologists have detected red blotch on less than 1% of the Classic vineyard crop. But it may not always be that way in the future.

“We don't know how long the Classic vineyard will remain clean,” Al Rwahnih said. “Every testing season, this is what keeps me up at night. We're not sure why it's happening in Russell Ranch and not the Classic vineyard.”

Once the greenhouse is operating, grapevines propagated from plant material from the Classic vineyard will be moved into the greenhouse, tested and verified as clean from disease. From there it will be sold to nurseries, which will grow additional plants to sell to growers.

Two greenhouses part of plan

Normally the foundation has 4,000 vines available, but the greenhouse will only house 2,000 vines, so inventory will be cut in half.

“This phase is just a starting phase,” Al Rwahnih said. “It's not sufficient for our needs.”

FPS plans to build another greenhouse in the next two to three years to increase capacity.

Industry groups and FPS identified greenhouses as the best way to protect the plants from red blotch and other pathogens transmitted by insects. They are also consulting with those same people on the grape varieties to include in the greenhouse.

“We have a large selection, and we need to make sure all the varieties that are important to industry are contained,” he said.

The first greenhouse is expected to be finished by the end of 2023.

Funding for the first greenhouse is coming from a variety of sources. The California Fruit Tree, Nut Tree and Grapevine Improvement Advisory Board, managed by the California Department of Food and Agriculture, contributed $4 million to the project. The California Grape Rootstock Research Foundation gave $500,000, Foundation Plant Services with UC Davis is funding $450,000, and the California Grape Rootstock Commission gave $100,000.

“This is crucial for the grapevine industry, and we are very grateful for the support,” Al Rwahnih said.

Grape experts give workshops on drought preparedness, red blotch

Grape growers and other industry members interested in grape production and water management in vineyards are invited to UC Cooperative Extension's Grapevine Drought Preparedness Workshops.

The workshops will be held in person on Friday, March 4, in San Luis Obispo and Friday, April 1, in Hopland.

Registration is $50 and includes a full day of live instruction from UC Cooperative Extension viticulture and grapevine experts. Lunch will be provided.

For more information and to register, visit https://ucanr.edu/sites/ShortCourse17.

UC Davis Grapevine Red Blotch Disease Symposium

On Wednesday, March 16, UC Cooperative Extension and the UC Davis Department of Viticulture and Enology will host a Grapevine Red Blotch Disease Symposium 9 a.m.-3:30 p.m.

Red blotch disease in grapevines, which can dramatically reduce the value of winegrapes, harms plants by inhibiting photosynthesis in the leaves. Infected vines are unable to conduct water effectively, leaving sugar that is created by photosynthesis stuck in the leaves instead of in the berries.

This event will be presented both in person at the UC Davis Conference Center and livestreamed for those unable to attend in person. 

Presentations will cover the role of treehoppers, treatments, mitigation strategies, the impact of the disease on the composition of wine, and more. 

Registration is $250 for the in-person symposium at UC Davis and $150 for the livestream. An application for 3.5 CCE units has been submitted to California Department of Pesticide Regulation and is pending approval.

To see the agenda and to register, visit https://wineserver.ucdavis.edu/events/uc-davis-grapevine-red-blotch-disease-symposium.

Citrus threat target of $7 million multistate research project

UC ANR part of team led by Texas A&M AgriLife combating huanglongbing disease

Citrus greening, or huanglongbing disease (HLB), is the most devastating disease for orange and grapefruit trees in the U.S. Prevention and treatment methods have proven elusive, and a definitive cure does not exist.

Since HLB was detected in Florida in 2005, Florida's citrus production has fallen by 80%. Although there have been no HLB positive trees detected in commercial groves in California, more than 2,700 HLB positive trees have been detected on residential properties in the greater Los Angeles region.

“It is likely only a matter of time when the disease will spread to commercial fields, so our strategy in California is to try to eradicate the insect vector of the disease, Asian citrus psyllid,” said Greg Douhan, University of California Cooperative Extension citrus advisor for Tulare, Fresno and Madera counties.

Now, a public-private collaborative effort across Texas, California, Florida and Indiana will draw on prior successes in research and innovation to advance new, environmentally friendly and commercially viable control strategies for huanglongbing.

Led by scientists from Texas A&M AgriLife Research, the team includes three UC Agriculture and Natural Resources experts: Douhan; Sonia Rios, UCCE subtropical horticulture advisor for Riverside and San Diego counties; and Ben Faber, UCCE advisor for Ventura, Santa Barbara and San Luis Obispo counties.

$7 million USDA project

The $7 million, four-year AgriLife Research project is part of an $11 million suite of grants from the U.S. Department of Agriculture National Institute of Food and Agriculture, NIFA, to combat HLB. The coordinated agricultural project is also a NIFA Center of Excellence.

“Through multistate, interdisciplinary collaborations among universities, regulatory affairs consultants, state and federal agencies, and the citrus industry, we will pursue advanced testing and commercialization of promising therapies and extend outcomes to stakeholders,” said lead investigator Kranthi Mandadi, an AgriLife Research scientist at Weslaco and associate professor in the Department of Plant Pathology and Microbiology at the Texas A&M College of Agriculture and Life Sciences.

The UC ANR members of this collaboration will be responsible for sharing findings from the research with local citrus growers across Southern California, the desert region, the coastal region and the San Joaquin Valley.

“In addition to the ground-breaking research that will be taking place, this project will also help us continue to generate awareness and outreach and share the advancements taking place in the research that is currently being done to help protect California's citrus industry,” said Rios, the project's lead principal investigator in California.

Other institutions on the team include Texas A&M University-Kingsville Citrus Center, University of Florida, Southern Gardens Citrus, Purdue University and USDA Agricultural Research Service.  

“This collaboration is an inspiring example of how research, industry, extension and outreach can create solutions that benefit everyone,” said Patrick J. Stover, vice chancellor of Texas A&M AgriLife, dean of the Texas A&M College of Agriculture and Life Sciences and director of Texas A&M AgriLife Research.  

HLB solutions must overcome known challenges

An effective HLB treatment must avoid numerous pitfalls, Mandadi explained.

One major problem is getting a treatment to the infected inner parts of the tree. The disease-causing bacteria only infect a network of cells called the phloem, which distributes nutrients throughout a tree. Starved of nutrients, infected trees bear low-quality fruits and have shortened lifespans.

Treatments must reach the phloem to kill the bacteria. So, spraying treatments on leaves has little chance of success because citrus leaves' waxy coating usually prevents the treatments from penetrating.

Second, while the bacteria thrive in phloem, they do not grow in a petri dish. Until recently, scientists wishing to test treatments could only do so in living trees, in a slow and laborious process.

Third, orange and grapefruit trees are quite susceptible to the disease-causing bacteria and do not build immunity on their own. Strict quarantines are in place. Treatments must be tested in groves that are already infected.

Two types of potential HLB therapies will be tested using novel technologies

The teams will be working to advance two main types of treatment, employing technologies they've developed in the past to overcome the problems mentioned above.

First, a few years ago, Mandadi and his colleagues discovered a way to propagate the HLB-causing bacteria in the lab. This method involves growing the bacteria in tiny, root-like structures developed from infected trees. The team will use this so-called “hairy roots” method to screen treatments much faster than would be possible in citrus trees.

In these hairy roots, the team will test short chains of amino acids – peptides – that make spinach naturally resistant to HLB. After initial testing, the most promising spinach peptides will undergo testing in field trees. To get these peptides to the phloem of a tree, their gene sequences will be engineered into a special, benign citrus tristeza virus vector developed at the University of Florida. The citrus tristeza virus naturally resides in the phloem and can deliver the peptides where they can be effective.

“Even though a particular peptide may have efficacy in the lab, we won't know if it will be expressed in sufficient levels in a tree and for enough time to kill the bacteria,” Mandadi said. “Viruses are smart, and sometimes they throw the peptide out. Field trials are crucial.”

The second type of treatment to undergo testing is synthetic or naturally occurring small molecules that may kill HLB-causing bacteria. Again, Mandadi's team will screen the molecules in hairy roots. A multistate team will further test the efficacy of the most promising molecules by injecting them into trunks of infected trees in the field.

A feasible HLB treatment is effective and profitable

Another hurdle to overcome is ensuring that growers and consumers accept the products the team develops.

“We have to convince producers that the use of therapies is profitable and consumers that the fruit from treated trees would be safe to eat,” Mandadi said.

Therefore, a multistate economics and marketing team will conduct studies to determine the extent of economic benefits to citrus growers. In addition, a multistate extension and outreach team will use diverse outlets to disseminate project information to stakeholders. This team will also survey growers to gauge how likely they are to try the treatments.

“The research team will be informed by those surveys,” Mandadi said. “We will also engage a project advisory board of representatives from academia, universities, state and federal agencies, industry, and growers. While we are doing the science, the advisory board will provide guidance on both the technical and practical aspects of the project.”

Project team members:

—Kranthi Mandadi, Texas A&M AgriLife Research.     

—Mike Irey, Southern Gardens Citrus, Florida.    

—Choaa El-Mohtar, University of Florida Institute of Food and Agricultural Sciences, Citrus Research and Education Center.

—Ray Yokomi, USDA-Agricultural Research Service, Parlier, California.

—Ute Albrecht, University of Florida IFAS Southwest Florida Research and Education Center.   

—Veronica Ancona, Texas A&M University-Kingsville Citrus Center.

—Freddy Ibanez-Carrasco, Texas A&M AgriLife Research, Department of Entomology, Weslaco.

—Sonia Irigoyen, AgriLife Research, Texas A&M AgriLife Research and Extension Center at Weslaco.

—Ariel Singerman, University of Florida IFAS Citrus Research and Education Center.

—Jinha Jung, Purdue University, Indiana.     

—Juan Enciso, Texas A&M AgriLife Research, Department of Biological and Agricultural Engineering, Weslaco.

—Samuel Zapata, Texas A&M AgriLife Extension, Department of Agricultural Economics, Weslaco.

—Olufemi Alabi, Texas A&M AgriLife Extension, Department of Plant Pathology and Microbiology, Weslaco.

—Sonia Rios, University of California Cooperative Extension, Riverside and San Diego counties.

—Ben Faber, University of California Cooperative Extension, Ventura, Santa Barbara and San Luis Obispo counties.

—Greg Douhan, University of California Cooperative Extension, Tulare, Fresno and Madera counties.

 

UC Davis releases 5 grape varieties resistant to Pierce’s disease

For the first time since the 1980s, University of California, Davis, researchers have released new varieties of wine grapes. The five new varieties, three red and two white, are highly resistant to Pierce's disease, which costs California grape growers more than $100 million a year. The new, traditionally bred varieties also produce high-quality fruit and wine.

“People that have tasted the wine made from these varieties are extremely excited,” said Andrew Walker, geneticist and professor of viticulture and enology at UC Davis, who developed the new Pierce's disease-resistant varieties. “They are impressed that they're resistant, but also that they make good wine.”

Pierce's disease a growing threat

Pierce's disease is caused by a bacterium spread by a group of insects called sharpshooters. It causes grapevine leaves to yellow or “scorch” and drop from the vine. The grape clusters also dehydrate, and infected vines soon die. While the disease has been around since the beginning of wine grape production in California, concerns have escalated with the arrival of the nonnative glassy-winged sharpshooter, which has the potential to spread the disease more rapidly. Pierce's disease occurs most often near rivers and creeks, and around urban and rural landscaping where sharpshooter populations reside. 

Pierce's disease also threatens wine grapes in the southeastern U.S. Rising temperatures from climate change could increase the spread of the disease, which is thought to be limited by cold winters. Growers in the Southeast can usually only grow Pierce's disease resistant varieties that don't have the same wine quality as the European winegrape species, Vitis vinifera, which is typically grown in California.

New varieties more sustainable

To create the new varieties, Walker crossed a grapevine species from the southwestern U.S. and northern Mexico, Vitis arizonica, which carries a single dominant gene for resistance to Pierce's disease and was used to cross back to Vitis vinifera over four to five generations. It's taken about 20 years to develop the five patent-pending selections that are now being released.

“These varieties will hopefully make viticulture much more sustainable and provide a high-quality wine that the industry will welcome,” said Walker. “So far there has not been tremendous interest in new wine grape varieties, but climate change may encourage growers to reconsider wine grape breeding as we work to address future climates and diseases.”

Winemaker Adam Tolmach, owner of The Ojai Vineyard in Ojai, planted four of the new varieties as part of a 1.2-acre experimental field trial. The trial was on the same plot of land where Pierce's disease wiped out his grapes in 1995. The vineyard then and now is organic, so spraying insecticides to fight the disease spread wasn't an option.

“I wasn't interested in planting in that plot again until I heard about these new Pierce's disease-resistant grape varietals,” said Tolmach. “This year was the first harvest. We've just begun to evaluate the wine but I'm very encouraged.”

Five varieties to suit every taste

The five new varieties of wines were evaluated by sensory tasting panels. Tasters included leading industry winemakers and enologists in prominent wine-growing regions of California and Texas as well as regions in the southeastern U.S. 

“What I think is exciting is that they're stand-alone varieties independent of whether they have Pierce's disease resistance,” said Doug Fletcher, former vice president of winemaking for Terlato Family Wine Group.

The three new red varieties are camminare noir, paseante noir and errante noir.

Camminare noir has characteristics of both cabernet sauvignon and petite sirah. The selection has ranked highly at numerous tastings of fruit grown in both Napa and Davis. Tasting comments: dark-red purple color, bright red fruit, raspberry, cherry, ripe, tannic, elegant rather than dense. The variety is 50% petite sirah and 25% cabernet sauvignon.

Paseante noir is similar to zinfandel. It has also been ranked highly at tastings. Tasting comments: medium dark red with purple; berry pie, cassis, black olive, herbal, dried hay, coffee, vegetal like cabernet sauvignon, licorice, round, moderate tannins, soft finish. The variety is 50% zinfandel, 25% petite sirah and 12.5% cabernet sauvignon.

Errante noir is a red winegrape most similar to a cabernet sauvignon and has great blending potential. Tasting comments: dark-red purple color; complex fruit with herbs and earth, plum, big wine, dense, rich middle, tannic yet balanced. The variety is 50% sylvaner and 12.5% each of cabernet sauvignon, carignane and chardonnay.

The two new white grape varieties are ambulo blanc and caminante blanc.

Ambulo blanc is similar to sauvignon blanc and has been tested in Temecula, Sonoma and along the Napa River. Tasting comments: light straw to clear color, citrus, lime, tropical, gooseberry, golden delicious apple flavors; bright fruit, slightly bitter, textured. The variety is 62.5% cabernet sauvignon, 12.5% carignane and 12.5% chardonnay.

Caminante blanc has characteristics of sauvignon blanc and chardonnay. Wines have been made from Davis fruit and ranked well. Field trials are underway at Pierce's disease hot spots in Ojai and Napa. Tasting comments: light straw-gold color, apple-melon, lychee, floral aromas, pineapple, green apple, juicy, harmonious, well-balanced. The variety is 62.5% cabernet sauvignon, 12.5% chardonnay and 12.5% carignane.

These five varieties are ready for patenting and release. There will be limited amounts of plant material available for propagation in 2020 as only a few of the grape nurseries participated in a pre-release multiplication program. Much more will be available in 2021. The Pierce's disease resistance breeding program continues, and more selections are approaching release.

 

Summer safety: Tips to avoid foodborne and heat illnesses

Happy summer! It's time to get the barbecue grilling and the pool party started. To keep your summer healthy and fun, UC ANR offers some important safety tips.

Food safety

Food poisoning is a serious health threat in the United States, especially during the hot summer months. According to the Centers for Disease Control and Prevention (CDC), 1 in 6 Americans suffer from a foodborne illness each year, resulting in thousands of hospitalizations and 3,000 deaths. 

Both the CDC and U.S. Department of Agriculture (USDA) suggest four key rules to follow to stay food safe:

• Clean: Clean kitchen surfaces, utensils, and hands with soap and water while preparing food. Wash fresh fruits and vegetables thoroughly under running water.
• Separate: Separate raw meats from other foods by using different cutting boards. And be sure to keep raw meat, poultry, seafood and eggs away from other items in your refrigerator.
• Cook: Cook foods to the right temperature; be sure to check internal temperature by using a food thermometer.
• Chill: Chill raw and prepared foods promptly.

Here are some additional tips from the USDA. Be sure to check out the CDC's comprehensive food safety website, which also has materials in both Spanish and English. For food safety tips in real time, follow USDA Food Safety on Twitter.

Summer also means more outside grilling, which can pose unique food safety concerns. Before firing up the barbecue, check out these five easy tips from UC Davis.

Before you take off on a road trip, camping adventure or boating excursion, don't forget to consider food safety. You'll need to plan ahead and invest in a good cooler.

Remember, warns the USDA, don't let food sit out for more than one hour in temperatures above 90 degrees F. And discard any food left out more than two hours; after only one hour in temperatures above 90 degrees F.

If there are any doubts about how long the food was out, it is best to throw it out!

Get more food safety tips for traveling from the USDA.

Avoid heat illness

“Summer can be a time for fun and relaxation, but in warm climates, we need to stay aware of the signs of heat illness and help keep our family members and co-workers safe,” says Brian Oatman, director of Risk & Safety Services at UC ANR.

“UC ANR provides comprehensive resources on our website, but it's designed around California requirements for workplace safety.” But, Oatman notes, much of the information applies.

“The training and basic guidance – drink water, take a rest when you are feeling any symptoms and having a shaded area available – are useful for anyone at any time.”

To increase your awareness of heat illness symptoms – and to learn more about prevention – Oatman suggests a few resources.

“Our Heat Illness Prevention page has many resources, including links for training, heat illness prevention plans, and links to other sites. One of the external sites for heat illness that I recommend is the Cal/OSHA site, which spells out the basic requirements for heat illness prevention in the workplace. It's also available in Spanish." 

For those on the go, Oatman also recommends the National Institute for Occupational Safety and Health (NIOSH) mobile heat safety app.

 

Read more